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• PCSK9 is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism.
• PCSK9 binds to the receptor for LDL, if PCSK9 is blocked, more LDLRs are recycled and are present on the surface of cells to remove LDL-particles from the extracellular fluid.
• The genome of mouse Pcsk9 gene including 5’UTR and 3’UTR was replaced by human PCSK9 genome including 5’UTR and 3’UTR in B-hPCSK9 mice plus.
• Mouse and human PCSK9 were detectable in heterozygous B-hPCSK9 mice plus.
• This product is used for pharmacodynamics and safety evaluation of hypercholesterolemia and other metabolic diseases.

Strain specific analysis of PCSK9 gene expression in wild-type mice and B-hPCSK9 mice plus by RT-PCR. Liver was collected from wild-type mice and heterozygous B-hPCSK9 mice plus. Human PCSK9 mRNA was detectable only in heterozygous B-hPCSK9 mice plus (H/+) but not in wild-type mice (+/+).

Strain specific PCSK9 expression analysis in heterozygous humanized B-hPCSK9 mice plus by ELISA. Serum was collected from heterozygous B-hPCSK9 mice plus (H/+) (n=1). Protein expression level of PCSK9 was analyzed by ELISA. Mouse PCSK9 and human PCSK9 were detectable in heterozygous B-hPCSK9 mice plus (H/+).