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    B-hHSD17B1-luc mice

    C57BL/6N-Gt(ROSA)Sortm1(CAG-HSD17B1, -luc)Bcgen/Bcgen • 113541

    B-hHSD17B1-luc mice

    Product nameB-hHSD17B1-luc mice
    Catalog number113541
    Strain nameC57BL/6N-Gt(ROSA)Sortm1(CAG-HSD17B1, -luc)Bcgen/Bcgen
    Strain backgroundC57BL/6N
    NCBI gene ID15485 (Human)
    AliasesE2DH; 17HSDB1; Hsd17ba; 17beta-HSD

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    • Description
    • Targeting strategy
    • Phenotypic analysis

    海报

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      发表文章

        Description
        • Hydroxysteroid (17-beta) dehydrogenase type 1 (HSD17B1) converts low-active estrogen estrone to highly active estradiol.
        • Postpubertal mammary gland development is extensively hormonally regulated, and 17-beta-estradiol (E2) is a hormone essential for normal postpubertal mammary gland development.
        • The full coding sequences of human HSD17B1 gene that is driven by CAG promoter are inserted into mouse Gt(ROSA)26Sor locus in B-hHSD17B1-Luc mice.
        • Human HSD17B1 was exclusively detected in uterus and ovary from heterozygous B-hHSD17B1-Luc mice but not in wild-type mice.
        • Heterozygous B-hHSD17B1-Luc mice can not conceive.
        • Humanized mice can be used to study the in vivo for cancer.
        Targeting strategy

        Gene targeting strategy for B-hHSD17B1-Luc mice. The full coding sequences of human HSD17B1 gene that is driven by CAG promoter are inserted into mouse Gt(ROSA)26Sor locus in B-hHSD17B1-Luc mice.

        Protein expression analysis

        Western blot analysis of HSD17B1 protein expression in heterozygous B-hHSD17B1-Luc mice. Various tissue lysates were collected from wild-type C57BL/6N (+/+) mice and heterozygous B-hHSD17B1-Luc mice (H/+), and then analyzed by western blot with anti-hHSD17B1 antibody (Abcam, ab51045). 40 μg total proteins were loaded for western blotting analysis. Human HSD17B1 was exclusively detected in uterus and ovary from heterozygous B-hHSD17B1-Luc mice but not in wild-type mice.

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hHSD17B1-luc mice] (Cat# 113541) was purchased from Biocytogen.