1. 德晋贵宾厅

      B-Gfap-iCre mice

      C57BL/6-Gfaptm1(icre)Bcgen/Bcgen • 110144

      B-Gfap-iCre mice

      Product nameB-Gfap-iCre mice
      Catalog number110144
      Strain nameC57BL/6-Gfaptm1(icre)Bcgen/Bcgen
      Strain backgroundC57BL/6
      NCBI gene ID14580 (Mouse)
      Official symbolGfap (glialfibrillary acidic protein)
      Chromosome11
      ApplicationThis Gfap-iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of astrocytes cells related signaling.

      在此页面上

      • Description
      • Targeting strategy
      • Phenotypic analysis

      海报

      查看全部

        发表文章

          Description


          This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

          Gene editing strategy
          A target vector was designed to insert iCre-WPRE-PA cassette into mouse endogenous genes 5 'UTR downstream of GFAP. The targeting vector was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulted chimeric mice. These mice were maintained on a C57BL/6 background.
          Phenotype analysis

          In situ hybridization in hippocampal CA1 area to confirm µR selective

          deletion from µRGFAP-/- mice with probes of µR mRNA (red),

          GAD2/ GFAP/vGlut1 mRNA (green), and DAPI nucleus staining (blue).

          * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Gfap-iCre mice] (Cat# 110144) was purchased from Biocytogen.