C57BL/6-Erbb3tm1(ERBB3)Bcgen/Bcgen • 110791
| Product name | B-hHER3 mice |
|---|---|
| Catalog number | 110791 |
| Strain name | C57BL/6-Erbb3tm1(ERBB3)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 2065 (Human) |
| Aliases | HER3; FERLK; LCCS2; VSCN1; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3 |
Gene targeting strategy for B-hHER3 mice. The exons 1-17 of mouse Her3 gene that encode the extracellular domain and transmembrane domain were replaced by human HER3 exons 1-17 in B-hHER3 mice.
Strain specific analysis of HER3 gene expression in wild type (WT) mice and B-hHER3 mice by RT-PCR. Mouse Her3 mRNA was detectable only in liver of WT mice (+/+). Human HER3 mRNA was detectable only in homozygous B-hHER3 mice (H/H) but not in WT mice (+/+).
Immunohistochemical (IHC) analysis of HER3 expression in homozygous B-hHER3 mice. The liver and colon were collected from WT and homozygous B-hHER3 mice (H/H) and analyzed by IHC with anti-HER3 antibody. HER3 was detectable in WT mice and homozygous B-hHER3 mice due to the cross-reactivity of the antibody. The arrow indicates tissue cells with positive HER3 staining (brown).
