NOD.CB17-PrkdcscidIl2rgtm1BcgenThpotm1(THPO)Bcgen/Bcgen • 112480
| Product name | huHSC-B-NDG hTHPO mice |
|---|---|
| Catalog number | 112480 |
| Strain name | NOD.CB17-PrkdcscidIl2rgtm1BcgenThpotm1(THPO)Bcgen/Bcgen |
| Strain background | B-NDG |
| NCBI gene ID | 21832,16186,19090 (Human) |
| Aliases | Ml; Tpo; Mgdf; Mpllg; gc; p64; [g]c; CD132; gamma(c); p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs; DNA-PKcs |
| Application | Irradiation is not required to avoid damage to mice caused by irradiation |
Human CD34+ HSCs (5E5) were intravenously engrafted into wild-type B-NDG mice and homozygous B-NDG hTHPO mice (female, 6-week-old, n=20). B-NDG mice were treated with 2.0 Gy-irradiation. B-NDG hTHPO mice were not irradiated. Flow cytometric analysis of peripheral blood lymphocyte from the two mice after engraftment with human CD34+ HSCs were shown in the figure above. Results showed that the survival rate of B-NDG hTHPO mice was higher than that of B-NDG mice. The percentages of human CD45+ leukocytes, T cells, B cells, NK cells, myeloid cells, monocytes, macrophages and neutrophils in B-NDG hTHPO mice were similar to those in B-NDG mice, demonstrating that the humanized mouse model engrafted with human CD34+ HSCs was successfully constructed. Values are expressed as mean ± SEM. HSCs: hematopoietic stem cells.
