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• LILRB1,also known as, ILT2, LIR1 and CD85j is a cell surface protein expressed on immune cells that has a known function in inhibiting the immune response. The protein contains 4 IgC domains in the extracellular region and 4 intracellular ITIM domains. It is a member of the ILT family, which is made up of ILT1, ILT2, ILT3 and ILT4. ILT2 is most similar to ILT4. Known ligands of ILT2 include MHC-1 as well as non-classical MHC molecules such as HLA-F, HLA-G, HLA-B27 and UL18 (human CMV). The strongest known interactor of ILT2 in the human genome is HLA-G1, activate LILRB1 and transduce a suppressive signal, which inhibits the immune responses. LILRB1 is widely expressed on human immune cells, including B cells, monocytes and macrophages, dendritic cells and subsets of natural killer (NK) cells and T cells. HLA-G1 is widely expressed on the surface of various malignancies including breast, cervical, CRC, lung, gastric, pancreatic, thyroid and ovarian cancer cells as well as glioblastoma multiform, melanoma cells. Antibody binding to ILT2 inducing/enhancing an anti-tumor T-cell response, increasing T-cell proliferation, reducing cancer-induced suppressor myeloid activity, increasing natural killer cell cytotoxicity, increasing macrophage phagocytosis, increasing generation of M1 inflammatory macrophages, decreasing generation of M2 suppressor macrophages, increasing dendritic cell number in a tumor microenvironment, increasing dendritic cell activation, treating an HLA-G expressing cancer, and treating a MHC-I expressing cancer.
• The BAC clone CH17-49J22, which contains the full sequence of the human LILRB1 gene, was randomly inserted into B-Tg(hLILRB1) mice(C). Protein expression was driven by the human LILRB1 promoter.
• Human LILRB1 was detectable in B cells, T cells, and NK cells, dendritic cells, monocytes, and macrophages of B-Tg(hLILRB1) mice(C), but not in neutrophils.
• This product is used for the pharmacological evaluation of LILRB1-targeted drugs in oncology.

Gene targeting strategy for B-Tg(hLILRB1) mice(C) . The BAC clone CH17-49J22, which contains the full sequence of the human LILRB1 gene, was randomly inserted into B-Tg(hLILRB1) mice (C). Protein expression was driven by the human LILRB1 promoter.​

Strain specific LILRB1 expression analysis in B-Tg(hLILRB1) mice(C) by flow cytometry. Splenocytes were collected from B-Tg(hLILRB1) mice(C), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody (eBioscience™, 17-5129-41). Human LILRB1 was detectable in B cells, T cells, and NK cells of B-Tg(hLILRB1) mice(C).

Strain specific LILRB1 expression analysis in B-Tg(hLILRB1) mice(C) by flow cytometry. Splenocytes were collected from B-Tg(hLILRB1) mice(C), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody (eBioscience™, 17-5129-41). Human LILRB1 was detectable in dendritic cells, monocytes, and macrophages of B-Tg(hLILRB1) mice(C), but not in neutrophils.

Strain specific LILRB1 expression analysis in B-Tg(hLILRB1) mice(C) by flow cytometry. Blood cells were collected from B-Tg(hLILRB1) mice(C), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody (eBioscience™, 17-5129-41). Human LILRB1 was detectable in B cells, T cells, and NK cells of B-Tg(hLILRB1) mice(C).

Strain specific LILRB1 expression analysis in B-Tg(hLILRB1) mice(C) by flow cytometry. Blood cells were collected from B-Tg(hLILRB1) mice(C), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody (eBioscience™, 17-5129-41). Human LILRB1 was detectable in dendritic cells, monocytes, and macrophages of B-Tg(hLILRB1) mice(C), but not in neutrophils.

Strain specific LILRB1 expression analysis in B-Tg(hLILRB1) mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+) and B-Tg(hLILRB1) mice(C), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody. hLILRB1 was detectable in B cells, T cells and NK cells of B-Tg(hLILRB1) mice(C).

Strain specific LILRB1 expression analysis in B-Tg(hLILRB1) mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+) and B-Tg(hLILRB1) mice(C), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody. hLILRB1 was detectable in monocytes, macrophages, and DCs of B-Tg(hLILRB1) mice(C), but not in neutrophils.

Strain specific LILRB1 expression analysis in B-Tg(hLILRB1) mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+) and B-Tg(hLILRB1) mice(C), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody. hLILRB1 was detectable in B cells, T cells and NK cells of B-Tg(hLILRB1) mice(C).

Strain specific LILRB1 expression analysis in B-Tg(hLILRB1) mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+) and B-Tg(hLILRB1) mice(C), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody. hLILRB1 was detectable in monocytes, macrophages, and DCs of B-Tg(hLILRB1) mice(C), but not in neutrophils.

Strain specific LILRB1 expression analysis in B-Tg(hLILRB1) mice(C) by flow cytometry. Blood cells were collected from wild-type BALB/c mice (+/+) and B-Tg(hLILRB1) mice(C), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody. hLILRB1 was detectable in B cells, T cells(low) and NK cells of B-Tg(hLILRB1) mice(C).

Strain specific LILRB1 expression analysis in B-Tg(hLILRB1) mice(C) by flow cytometry. Blood cells were collected from wild-type BALB/c mice (+/+) and B-Tg(hLILRB1) mice(C), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody. hLILRB1 was detectable in monocytes, macrophages, and DCs of B-Tg(hLILRB1) mice(C), but not in neutrophils.