德晋贵宾厅

B-hSEMA4D mice

C57BL/6-Sema4dtm1(SEMA4D)Bcgen/Bcgen • 112334

B-hSEMA4D mice

Product nameB-hSEMA4D mice
Catalog number112334
Strain nameC57BL/6-Sema4dtm1(SEMA4D)Bcgen/Bcgen
Strain backgroundC57BL/6
NCBI gene ID10507 (Human)
AliasesA8; GR3; BB18; CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G

在此页面上

  • Targeting strategy
  • Phenotypic analysis

海报

查看全部

    发表文章

      Targeting strategy

      Gene targeting strategy for B-hSEMA4D mice. The chimeric SEMA4D CDS (replace the extracellular domain) was inserted in the exon 4 of mouse Sema4d gene in B-hSEMA4D mice.

      Protein expression analysis (T cells)

      Strain specific SEMA4D expression analysis in T cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

      Protein expression analysis (B cells)

      Strain specific SEMA4D expression analysis in B cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

      Protein expression analysis (NK cells)
      Strain specific SEMA4D expression analysis in NK cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
      Protein expression analysis (DC cells)
      Strain specific SEMA4D expression analysis in DC cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
      Protein expression analysis (granulocytes)
      Strain specific SEMA4D expression analysis in granulocytes of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
      Protein expression analysis (macrophages)
      Strain specific SEMA4D expression analysis in macrophages of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
      Protein expression analysis (monocytes)

      Strain specific SEMA4D expression analysis in monocytes of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hSEMA4D mice] (Cat# 112334) was purchased from Biocytogen.