德晋贵宾厅

• This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A.
• The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor.
• The human gene coding sequence was inserted to mouse Hipp11(H11) allele in B-hMICA mice. Human MICA was exclusively detectable on T cells, B cells and NK cells of homozygous B-hMICA mice.
• Application: For example, This product is used for pharmacodynamics and safety evaluation of cancer.

Strain specific MICA expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hMICA mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hMICA mice (H/H), stimulated with PMA+ionomycin cocktail in vitro ( Cells were cultured in presence of PMA/I for 2 h, extensively washed, and subsequently cultured for up to 22 h) and analyzed by flow cytometry with species-specific MICA/MICB antibody. Human MICA was exclusively detectable on T cells, B cells and NK cells of homozygous B-hMICA mice.