C57BL/6N-Olr1tm2(OLR1)Bcgen/Bcgen • 111978
| Product name | B-hLOX1 mice |
|---|---|
| Catalog number | 111978 |
| Strain name | C57BL/6N-Olr1tm2(OLR1)Bcgen/Bcgen |
| Strain background | C57BL/6N |
| NCBI gene ID | 4973 (Human) |
| Aliases | LOX1; LOXIN; SLOX1; CLEC8A; SCARE1 |
Strain specific analysis of LOX1 mRNA expression in wild-type C57BL/6 mice and B-hLOX1 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hLOX1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LOX1 primers. Mouse Lox1 mRNA was detectable only in wild-type C57BL/6 mice. Human LOX1 mRNA was detectable only in homozygous B-hLOX1 mice but not in wild-type mice. And the PCR products were confirmed with Sanger sequencing.
Strain specific LOX1 expression analysis in homozygous B-hLOX1 mice by flow cytometry. Bone marrow-derived dendritic cells(BMDC) were cultured from wild-type C57BL/6 mice (+/+) and homozygous B-hLOX1 mice (H/H) stimulated with LPS (1μg/mL , stimulation for 18 hour), and analyzed by flow cytometry with species-specific anti-LOX1 antibody. Mouse LOX1 was detectable on BMDC of wild-type mice. Human LOX1 was detectable on BMDC of homozygous B-hLOX1 mice.
