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    B-hIL15/hIL15RA mice
    
                
    C57BL/6-Il15tm1(IL15)BcgenIl15ratm1(IL15RA)Bcgen/Bcgen • 121217
    
              
    
            
    
            
          
    
        
    
      
    
    
    

    

    
    
    
      
    
        
    
          
    
            
    
              
            
    
                      
    
          
    B-hIL15/hIL15RA mice
    
        
    
        
    
          
    
            
    
              
    
                
    
                  
    
                    
                      
    
                        
                        
                      
    
                      
    Product nameB-hIL15/hIL15RA mice
    
                      
    Catalog number121217
    
                                            
    Strain nameC57BL/6-Il15tm1(IL15)BcgenIl15ratm1(IL15RA)Bcgen/Bcgen
    
                                            
    Strain backgroundC57BL/6
    
                                            
    NCBI gene ID3600,3601 (Human)
    
                                            
    AliasesIL-15; CD215
    
                                            
    
                        
                        
                      
    
                        
                  
    
                
    
              
    
              
            
    
          
    
        
    
      
    
    
    
    
    
    
      
      
    
        
    
          
    
            
    
              
    在此页面上
    
              
      
                                
    • Targeting strategy
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    • Phenotypic analysis
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    • Efficacy
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    • Related products
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        Targeting strategy
        
              
        
                            
        
                            
        Gene targeting strategy for B-hIL15/hIL15RA mice. The mouse Il15 gene that encodes the full coding sequence was replaced by human IL15 counterpart gene sequences in B-hIL15/hIL15RA mice. The mouse Il15ra gene that encodes the extracellular region was replaced by human IL15RA counterpart gene sequences in B-hIL15/hIL15RA mice.
        
                          
        
                                    
        
                            
        
                                
        
                                
        mRNA expression analysis
        
              
        
                            
        
                            
        
                
              
        
                            
        Strain specific analysis of IL15 and IL15RA gene expression in wild-type mice and B-hIL15/hIL15RA mice by RT-PCR. Mouse Il15 and Il15ra mRNA were detectable in spleen of wild-type mice (+/+) but not in homozygous B-hIL15/hIL15RA mice (H/H). Human IL15 and IL15RA mRNA were detectable only in homozygous B-hIL15/hIL15RA mice but not in wild-type mice.
        
                            
        
                                
        
                                
        Protein expression analysis
        
              
        
                            
        
                            
        
                
              
        
                            
        Strain specific IL15 expression analysis in homozygous B-hIL15/hIL15RA mice by ELISA. (A) Mouse IL15; (B) Human IL15; Serum was collected from wild-type mice (+/+) and homozygous B-hIL15/hIL15RA mice (H/H), and analyzed by ELISA with IL15 ELISA kit. Human IL15 was only detectable in homozygous B-hIL15/hIL15RA mice but not in wild-type mice. Mouse IL15 was both detectable in wild-type mice and homozygous B-hIL15/hIL15RA mice. According to that mouse IL15 was not detection in homozygous B-hIL15/hIL15RA mice in mRNA level, probably the ELISA kit has cross reaction between human and mouse.
        
                            
        
                            
        
                
              
        
                            
        IL15RA expression analysis in homozygous B-hIL15/hIL15RA mice by flow cytometry. Dendritic cells were collected from wild-type mice (+/+) and homozygous B-hIL15/hIL15RA mice (H/H),and analyzed by flow cytometry with species-specific anti-IL15RA antibody. Mouse IL15RA was detectable in wild-type mice but not in homozygous B-hIL15/hIL15RA mice. Human IL15RA was exclusively detectable in homozygous B-hIL15/hIL15RA mice but not in wild-type mice.
        
                            
        
                                
        
                                
        Analysis of spleen leukocytes subpopulation
        
              
        
                            
        
                            
        
                
              
        
                            
        Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hIL15/hIL15RA mice (n=3, female, 8-week-old). Flow cytometry analysis of the splenocytes were performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hIL15/hIL15RA mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
        
                            
        
                                
        
                                
        Analysis of spleen T leukocytes subpopulation
        
              
        
                            
        
                            
        
                
              
        
                            
        Analysis of T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hIL15/hIL15RA mice (n=3, female, 8-week-old). The proportion of T cells subpopulation was tested by flow cytometry. A. Representative FACS plots. Single live cells were gated for the CD3+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD4+ T cells, CD8+ T cells and Tregs in homozygous B-hIL15/hIL15RA mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
        
                            
        
                                
        
                                
        Analysis of blood leukocytes subpopulation
        
              
        
                            
        
                            
        
                
              
        
                            
        Analysis of blood leukocyte subpopulations by FACS. Blood cells were isolated from female C57BL/6 and B-hIL15/hIL15RA mice (n=3, female, 8-week-old). Flow cytometry analysis of the blood cells were performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hIL15/hIL15RA mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
        
                            
        
                                
        
                                
        Analysis of blood T leukocytes subpopulation
        
              
        
                            
        
                            
        
                
              
        
                            
        Analysis of T cell subpopulations by FACS. Blood cells were isolated from female C57BL/6 and B-hIL15/hIL15RA mice (n=3, female, 8-week-old). The proportion of T cells subpopulation was tested by flow cytometry. A. Representative FACS plots. Single live cells were gated for the CD3+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD4+ T cells, CD8+ T cells and Tregs in homozygous B-hIL15/hIL15RA mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
        
                            
        
                                
        
                                
        Analysis of lymph node leukocytes subpopulation
        
              
        
                            
        
                            
        
                
              
        
                            
        Analysis of lymph node leukocyte subpopulations by FACS. Lymph node cells were isolated from female C57BL/6 and B-hIL15/hIL15RA mice (n=3, female, 8-week-old). Flow cytometry analysis of the lymph node cells were performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, CD4+ T cells and CD8+ T cells in homozygous B-hIL15/hIL15RA mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
        
                            
        
                                
        
                                
        Analysis of lymph node T leukocytes subpopulation
        
              
        
                            
        
                            
        
                
              
        
                            
        Analysis of T cell subpopulations by FACS. Lymph node cells were isolated from female C57BL/6 and B-hIL15/hIL15RA mice (n=3, female, 8-week-old). The proportion of T cells subpopulation was tested by flow cytometry. A. Representative FACS plots. Single live cells were gated for the CD3+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD4+ T cells, CD8+ T cells and Tregs in homozygous B-hIL15/hIL15RA mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
        
                          
        
                                    
        
                            
        
                                
        
                                
        Effects of anti-human IL15 antibody in psoriasis model of B-hIL15/hIL15RA mice
        
              
        
                            
        
                            
        
                
              
        
                            
        Effects of AMG-714 (in house) in psoriasis-like skin lesions model of B-hIL15/hIL15RA mice. (A-B) Body weight changes during treatment. (C-D) Erythema and the scaling score of the back were scored daily from 0 to 6. Additionally, the total score (erythema plus scaling) is depicted (E). Results indicated that structural features of IMQ-induced skin inflammation is visible and increased in severity up to day 4. 3 mg/kg Anti-human IL15 antibody improve the disease score of erythema and scaling, but no effective in body weight.
        
                          
        
                                    
        
                            
        
                                
        
                                
        Target-related cell line and animal products
        
              
        
                            
        
                            
        
                
              
        
                            
        
                          
        
                                                
        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hIL15/hIL15RA mice] (Cat# 121217) was purchased from Biocytogen.