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    B-Luc-EGFP HL-60

    • 321821

    B-Luc-EGFP HL-60

    Product nameB-Luc-EGFP HL-60
    Catalog number321821
    AliasesNA
    TissuePeripheral blood
    DiseaseAcute myelogenous leukemia
    SpeciesHuman
    ApplicationB-Luc-EGFP HL-60 cell line can be used to evaluate anti-cancer drugs in acute myelogenous leukemia

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    • Description
    • Targeting strategy
    • Phenotypic analysis
    • Tumorigenicity

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      发表文章

        Description

        This B-Luc-EGFP HL-60 cell line expresses firefly luciferase as a marker of HL-60 cells. Fluorescence could be detected in B-Luc-EGFP HL-60 cells.

        Targeting strategy

        Gene targeting strategy for B-Luc-EGFP HL-60 cells. The CMV promoter and luciferase-EGFP were inserted into the targeting vector used in the lentivirus system.

        In vitro luciferase activity of B-Luc-EGFP HL-60 cells

        Luminescence signal intensity of B-Luc-EGFP HL-60 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay. B-Luc-EGFP HL-60 cells have a strong luminescence signal. EGFP was detected in B-Luc-EGFP HL-60 cells. The 1-B06 clone of B-Luc-EGFP HL-60 cells can be used in vivo experiments.

        Quantitative imaging data & body weight changes

        B-Luc-EGFP HL-60 cells (1x105) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc-EGFP HL-60 cells can be used for in vivo efficacy evaluation.