• 310724
| Product name | B-hHER2 EL4 |
|---|---|
| Catalog number | 310724 |
| Strain background | C57BL/6N |
| NCBI gene ID | 13866 (Human) |
| Aliases | Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; l11Jus8; mKIAA3023 |
| Tissue | T lymphocyte |
| Disease | Lymphoma |
| Species | Mouse |
| Application | B-hHER2 EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies. |
The mouse Her2 gene was replaced by human HER2 coding sequence in B-hHER2 EL4 cells. Human HER2 is highly expressed on the surface of B-hHER2 EL4 cells.
Gene targeting strategy for B-hHER2 EL4 cells. The exogenous promoter and human HER2 coding sequence were inserted to replace part of murine exon 2 and all of exons 3~7. The insertion disrupts the endogenous murine Her2 gene, resulting in a non-functional transcript.
HER2 expression analysis in B-hHER2 EL4 cells by flow cytometry. Single cell suspensions from wild-type EL4 and B-hHER2 EL4 cultures were stained with species-specific anti-human HER2 antibody. Human HER2 was detected on the surface of B-hHER2 EL4 cells, but not on the surface of wild-type EL4 cells. The 2-H01 clone of B-hHER2 EL4 cells was used for in vivo experiments.
Subcutaneous homograft tumor growth of B-hHER2 EL4 cells. B-hHER2 EL4 cells (2x105) and wild-type EL4 cells (2x105) were subcutaneously implanted into C57BL/6 mice (female, 6-9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean ± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHER2 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.
Subcutaneous homograft tumor growth of B-hHER2 EL4 cells. B-hHER2 EL4 cells and wild-type EL4 cells were subcutaneously implanted into B-h4-1BB mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHER2 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
B-hHER2 EL4 tumor growth of individual mice. B-hHER2 EL4 cells (2x105) and wild-type EL4 cells (2x105) were subcutaneously implanted into C57BL/6 mice (female, 6-9-week-old, n=5). As shown in panel, B-hHER2 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.
B-hHER2 EL4 tumor growth of individual mice. B-hHER2 EL4 cells and wild-type EL4 cells were subcutaneously implanted into B-h4-1BB mice (female, 8-week-old, n=6). As shown in panel, B-hHER2 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.
